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Putrescine levels were increased in vivo and in vitro in agreement with findings in patients with an overactive bladder 35

The stability of EVs is contingent upon a number of factors, including buffer concentration and pH value [180]

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The cycling conditions were: 98 C for 30 s, followed by 15 cycles of 98 C for 15 s, 63 C for 20 s, 72 C for 90 s, 25 cycles of 98 C for 15 s, 72 C for 15 s, 72 C for 90 s, followed by final extension at 72 C for 5 min

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