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J Clin Endocrinol Metab 91, 35483552 (2006)

174 StokerM.GherardiE.PerrymanM.GrayJ

Consequently, the state of abstinence could disrupt this equilibrium, and an increase in BDNF, concomitant with greater craving, could trigger a behavioral mechanism that seeks to reestablish this balance

Crystals were cryoprotected through the addition of 180 nL of 40% glycerol to the crystal drop prior to harvesting, giving a final glycerol concentration of 28.835.5% (accounting for the glycerol already in the crystallisation conditions)