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For comparison, a multi-dose treatment with Nrf2 activators such as isothiocyanate-containing broccoli seeds, sulforaphane, erucin, or else the potent sulforaphane analog 6-methylsulfinylhexyl isothiocyanate, was needed to achieve similar levels of induction in the cytosolic fraction of liver in adult mice, i.e

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The analysis revealed that Day 30 represents the peak of functional maturity, marked by the highest expression of key-metabolizing enzymes, such as cytochrome P450 3A4 (CYP3A4), along with cholangiocyte-specific proteins and hepatic metabolic enzymes