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coli ) [238]

doi: 10.1080/09513590.2018.1540573

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It involves gently stretching the spine to create negative pressure within the intervertebral discs, promoting nutrient exchange, and relieving pressure on compressed nerves

When the cells reached approximately 80% confluency, cells were harvested with trypsin and seeded at a density of 2.9 10 5 cells per well in 2.5 ml of culture medium into 6 well flat-bottomed plates (Costar, Corning, USA) and incubated for 24 hr