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Description

Serum protection assay Protection from complement-mediated lysis mediated by recombinant proteins was assessed by pre-incubation of 25 l NHS with Chi proteins, BGA66 of B

High production of ROS caused damage to the cells, including spermatozoa (De Lamirande and Gagnon, 1995)

Whole-cell current clamp recordings were made with a headstage (CV-7B) connected to a computer-controlled amplifier (MultiClamp 700B, Axon Instruments), digitized through a Digidata 1440A Analog/digital converter and acquired at a sampling rate of 10 kHz