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However, several critical gaps currently limit its translation into clinical practice: (1) the lack of sensitive and standardized biomarkers for early detection of bioenergetic deficits, (2) limited incorporation of creatine profiling into predictive risk models, (3) insufficient personalization of supplementation strategies despite known interindividual variability in transporter function, endogenous synthesis, and tissue kinetics, and (4) underdeveloped clinical validation of advanced creatine formulations and delivery systems

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In order to further prove the role of Caspase 3 in inducing pyroptosis, we performed Annexin V-FITC/PI double staining analysis to distinguish pyroptosis and apoptosis according to literature reports [52,53,54]

2.3.4 Glutamine synthetase Glutamine can be synthesized de novo from glutamate via glutamine synthase (GS)