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The medium was inoculated at an optical density of 0.2 (at 600 nm) and the cultures were incubated at 25 C, without shaking

To address this, researchers have investigated the toxic impact of NAg on environmental organisms, plant and animal models, and human cells, with studies still on-going to determine the toxicity threshold and long-term environmental and human health effects of NAg (Burduel et al., 2018