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The lysate was clarified by centrifugation at 15,000 nx g for 15 min, protein concentrations were determined using the BCA total protein assay (Pierce), and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten min at 95 C

Comparison of faster R-CNN, YOLO, and SSD for third molar angle detection in dental panoramic X-rays

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Morris AL, Mohiuddin SS

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The two procedures are distinct from each other