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Inhalation of O 2 concentrations higher than the normal partial pressure can lead to high reactive oxygen species (ROS) levels

Then, cell monolayer was covered with overlay medium comprised of 25 mL 4DMEM, 25 mL ddH 2 O, 50 mL 4% methycellulose and 2 mL FBS, and incubated at 37 in a humidified atmosphere of 5% CO 2

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Indeed, addition of excess Cu(II) ions significantly enhanced the in vitro toxicity of the studied chelators in A431 cells, resulting in approximately a three-fold elevation

Their contribution towards free radical scavenging, Osmo-protection, carbon storage, and maintenance of the structure of essential proteins like RUBISCO has been widely acknowledged

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