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These DAMs showing the highest variations, representing the main downregulated or upregulated metabolites across all samples, mainly belonged to the lipids, amino acids, peptides, analogs, carbohydrates, carbonyl compounds, phenols or flavonoids, indicating their significance as the characteristic metabolites

The cells were maintained at 37C in an atmosphere of 5% CO2 in RPMI-1640 (Thermo Fisher Scientific, Inc., Waltham, MA, USA) with 10% fetal bovine serum (FBS

Sasaki et al., 2017)

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Briefly, 5.0 10 5 cells were transfected with 1.5 g of DNA plasmid and 3 L of Lipofectamine 2000 in a 12-well plate setting according to the manufacturers instructions