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There are a number of possible reasons including 1) tandemly repeated genes such as NBS-LRR were not anchored well using the genetic linkage map markers, which only represent unique sequences in the genome, 2) high heterozygosity levels in the chestnut genome coupled with high sequence similarities among NBS-LLR genes may have caused the heterozygosity-option in the Newbler software to collapse the copy number of tandemly repeated genes during the assembly process

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In the time in the empty segment (Fig