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Then, the cells were washed and suspended in Roswell Park Memorial Institute-1640 medium (RPMI-1640, Sigma-Aldrich) supplemented with 2 g/L sodium bicarbonate, 10% fetal bovine serum (FBS, Sigma-Aldrich), 2% glutamine, and 100 U/mL penicillin-0.1 mg/mL streptomycin (Sigma-Aldrich) and incubated in vitro with RPMI medium (control), tert -Butyl hydroperoxide (tBHP: 200 M) or tBHP plus Ab (22.5 mg/mL) for 30 min at 37C

People around the world consume different amounts of quercetin daily: United States: 9.75 mg per day [1] Spain: 18.48 mg per day [1] Japan: 16.2 mg per day [1] China: 18 mg per day [1] Foods rich in quercetin include: Vegetables : Onions, asparagus, green peppers, broccoli, kale, and red leaf lettuce [3] Fruits : Apples, red grapes, berries (cranberries have up to 22 mg per 100g), cherries, and citrus fruits [4] Beverages : Green tea (2.1 mg per 100 mL), red wine (about 3 mg per 100 mL) [3] Others : Capers (highest amount per gram), buckwheat, olive oil, and various herbs [2] Cooking methods change quercetin levels

It is not supplied for human consumption, veterinary use, cosmetic use, diagnostic use, medical use, therapeutic use, supplementation, wellness use, performance enhancement, personal use, or administration to humans or animals

Round up for priming losses, dropped syringes, and protocol adjustments

JCI Insight 1 , e86326 (2016)