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The localization was determined using GAS7b tagged with mEOS4b, a photoconvertible fluorescent protein that enables stochastic observations for super-resolution imaging 33

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Other longevity peptides like epitalon or MOTS-c have more human-relevant data, even if their senolytic mechanisms are less direct

RNA was extracted from pelleted cell culture samples using the RNeasy Isolation kit (Qiagen) as per manufacturers instructions