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NFkB pathway is the major pathway of inflammation

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After centrifugation, the supernatants were added to 2.3 mL of deionised water, 100 mL of 0.3M EDTA, 300 mL of 0.32M tris(hydroxymethyl)aminomethane (TRIS) and 100 mL of 0.086 mM 5,5-dithiobis-2-nitrobenzoic acid (DTNB), and were maintained at 10 C for 10 min

Hyperactive neuronal autophagy depletes BDNF and impairs adult hippocampal neurogenesis in a corticosterone-induced mouse model of depression