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Mechanistic Research Perspective Oxidative stress pathway investigation Redox signaling and antioxidant defense research Detoxification and conjugation pathway analysis Mitochondrial function and metabolic signaling studies Cellular protection and stress response modeling Primary Research Applications Oxidative Stress & Redox Balance Research: Glutathione is widely used to model cellular responses to oxidative stress and investigate redox regulation mechanisms

10.1177/1534735419888584 110 MateosM

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Briefly, 1 mL of assay mixture contains optimized concentrations of the following chemicals: 0,5 M K 2 HPO 4 (pH 7.0), 2,5 mM EDTA, 0,18 U/mL GR, 100 mM glutathione and 10 mM reduced NADPH and tissue extract (0,5 mL) was added in the spectrophotometer cuvette along with 0,1 mL of 60 mM cumene hydroperoxide, a suitable substrate for GP