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neoformans GSH mutants had reduced migration to the brains of mice

Gesztes T

demonstrated that various subcellular membranes undergo lipid peroxidation during ferroptosis, and the resulting lipid peroxides accumulate initially in the endoplasmic reticulum (ER) membrane and later in the plasma membrane[30]

After electrophoresis, separated proteins were transferred onto a nitrocellulose membrane (VWR international, 10600003) in a wet-transfer apparatus (Bio-Rad, 350 mA, 90 min, 4 C) with buffer containing 2.5 mM TrisHCl, 10% (v/v) methanol and 19.2 mM glycine

NM_002084.3), in which the codons for Cys-12 (TGC), Cys-81 (TGC) and Cys-136 (TGT) were replaced with the codons for Ser (TCG) and the codon for Sec-53 (TGA) was changed to Cys codon (TGC)

h , Western blot analysis of immunoprecipitation using the M2 anti-Flag antibody in cells expressing wild-type or mutant FlagENL