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Considering that doubling times can affect cell phenotype and responses, cells were discarded after 30 population doublings from the initial culture provided by Coriell, and new cultures were started from aliquots frozen at low cell passage

Tryptophan degradation increases with stage in patients with rheumatoid arthritis

Aug 2009;93(2):128-34

Early administration of photobiomodulation significantly attenuates the accumulation of ROS, oligodendrocyte dysfunction, and compromised antioxidant defense, thereby preventing adverse neurological outcomes in a rat model of early life adversity, including cognitive dysfunction and depressive-like symptoms in adulthood [199]

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Additional experiments also need to be performed to eliminate the possibility of contraindications of any possible adjuvants with the ASMs