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The GSH/GSSG content and ATP levels of 0.51 10 5 cells/well were measured by a luminescent-based assay (Promega V6612 for GSH/GSSG and Promega G7571 or Sigma MAK135 for ATP) following the manufacturers protocol

16 , e8982 (2020)

Exploration of uncharted regions of the protein universe

Moreover, elevated levels of leptin, a prevalent occurrence in individuals with obesity, have been shown to stimulate the secretion of vascular endothelial growth factor (VEGF) by airway ASM cells (293), suggesting that leptin may indirectly contribute to the obesity-induced bronchial vascular remodeling processes in individuals with asthma and obesity

Compared with other flavonoids, Lut has a stronger research basis for its anti-asthmatic properties and has been shown to relieve a range of asthma symptoms, including airway inflammation [18], airway hyperresponsiveness, bronchoconstriction [19], airway mucus hypersecretion [20], and lung autophagy [21], as well as neutrophil asthma in OVA/LPS-stimulated asthmatic mice [22]

A., & Styczynski, M