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After a 4-hour incubation, the medium was removed and 1 mL dimethyl sulfoxide was added to each well for cell lysis

doi: 10.12688/f1000research 21 BertaG.CopettaA.GamaleroE.BonaE.CesaroP.ScarafoniA.et al

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After sonication, the lysate was centrifuged (17,000 rpm, 40 min) and the supernatant was purified using a nickel-nitrilotriacetic acid column (HisTrap HP

Several limitations and challenges must be addressed for the clinical translation of UA as follows