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(3) Ultrasonic extraction in an ice-water bath for 10 min, and leave at -40C overnight

Markovic J, Borras C, Ortega A, Sastre J, Vina J, et al

In the case of lipid superoxide this means that it cannot become an initiator of lipid peroxidation and, therefore, GSH-Px inhibits the lipid peroxidation process (31,37)