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These sequences were then run through an R script (Supplementary Materials, bash_DADA2 + IDTAXA_workflow_Illumina-PairedEnd_V1V3.R) which trimmed reverse reads to 260 bp, removed forward reads exceeding a maximum expected error of three, and truncated reverse reads exceeding a maximum expected error of six

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10.1038/s41467-023-38771-4 270 ZhangY.ZhangN.ZouY.SongC.CaoK.WuB.et al (2023d)