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Proteins from the gel were electroblotted onto nitrocellulose membranes (Schleicher and Schuell) at 100V for 1 h, using BioRad Mini Trans-Blot transfer cells

Following his Habilitation at LMU Munich, Hamdorf was appointed in 1969 as professor of visual physiology at the Ruhr-University of Bochum where he stayed until his retirement in 1994

However, in vitro, angiogenic cytokine treatment activates the nuclear translocation of NRF2, which stimulates the tube formation of endothelial cells

In vitro experiments further reveal that Rd rectifies vasopermeability of RVECs in response to high glucose through the reconstruction of adhering, tight and gap junctions

(2) We applied cryo-EM to demonstrate that, when incubated with liposomes, Arabidopsis MVB-localized, BAR domain-containing SNX proteins generate significantly shorter tubules than mouse SNX1 in vitro