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Thymol has been shown to modulate the expression of antioxidant enzymes both in vitro and in vivo across various ROS models, primarily by activating the Nrf2ARE antioxidant response element signaling pathway (66)

Zinc decreases C-reactive protein, lipid peroxidation, and inflammatory cytokines in elderly subjects: a potential implication of zinc as an atheroprotective agent

Alternatively, you can also use different species of antibody for blotting to avoid seeing the primary antibodies use for IP

The calibration and validation results for all cases are included in the Supplementary Information

Either of these strategies alone cannot lead to the desired upregulation of seleno-GPxs