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Zhang et al., 2024) identify GPX4 as a key enzyme counteracting LPO in hippocampal neurons, closely linked to AD neurodegeneration

The body tightly regulates intracellular glutathione levels along with its synthesis from amino acid precursors

The beads were washed four times with high-salt wash buffer (10 mM HEPES, pH 7.9, 25% glycerol, 1.5 mM MgCl 2 , 300 mM KCl, and 0.1% Triton X-100), followed by two washes with low-salt wash buffer (10 mM HEPES, pH 7.9, 25% glycerol, 1.5 mM MgCl 2 , 100 mM KCl, and 0.1% Triton X-100)

The parameters of a six-point calibration curve were used for glutathione concentration determination

& Rost, J

Moreover, caffeine has been shown to inhibit chronic inflammation, its anti-inflammatory nature downregulates the pro-inflammatory cytokines and microglial activation, thereby protecting neuronal damage (156)