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For direct enzyme immobilization from cell lysates, all three enzymes (d-PhgAT, HeT and BsGDH) showed activities (initial rates) comparable to the correspondent immobilized enzymes prepared via a conventional method (i.e

Based on the total intensity observed from each TMT channel in the labeling efficiency check, the remainder of each sample for each 10-plex was mixed such that total signal in each channel was approximately equal

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The mixed solution was dialyzed against water for approximately 12 h

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