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Effects of SAG on Pro-Inflammatory Mediator Secretion Induced by CCl4 Chronic Exposure ELISA analysis showed elevated levels of TNF- (Figure 5A), IL-6 (Figure 5B), IL-1 (Figure 5C) and MCP-1 (Figure 5D) in the liver after CCl4 intoxication, which were reduced by SAG administration

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We used 16S rRNA gene amplicon sequencing to assess the microbial composition and predicted metabolic pathways in ruminal solid and liquid digesta, and feces