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After thrombin cleavage (1 nM -thrombin overnight at 4 C) to remove the His 6 tag, the sample was applied to a Superdex-75 (GE Healthcare Life Sciences) equilibrated in NMR buffer (50 mM bis-tris, pH 6.5, 50 mM NaCl, 1 mM DTT)

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References Ask M (2013) Towards more robust Saccharomyces cerevisiae strains for lignocellulosic bioethanol production: lessons from process concepts and physiological investigations [Internet]