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Wachtel says he was visiting a friend, LeRoy Marinell, in London, when he received a call

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Enzyme activity assay To determine enzyme activity in cell lysates, approximately 10 million HL-60 cells were lysed using hypotonic protein assay buffer (25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, 5% glycerol, phosphatase inhibitor, 3 HALT protease inhibitor)

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