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Materials and Methods Principle of developed redox profiling method For quantitative analysis of NAD + , NADH, NADP + , NADPH, GSSG and GSH in a single blood sample, we developed a comprehensive method, NADMED, consisting of novel extraction coupled with detection based on previously published cyclic enzymatic reactions with colorimetric detection 23,24,3234 in a high-throughput 96-well-plate analysis

However, the function and mechanism of GPX3 in breast cancer have not been reported and need to be further elucidated

D.GoodwinP

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Cazzola M, Floriani I, Page CP

To break-out the region displaced by the ligand, the grid_comp command was run on the results of the previous step