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however as the disease progresses, pathogens in the microbiome disable the immune response and immune exhaustion occurs (233, 234)

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Therefore, it is recommended to use a combination of methods, such as flow cytometry with confocal laser scanning (CLS) microscopy [86] or stereological sampling techniques with transmission electron microscopy [87]

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Since the hallmark of SCD is chronic hemolysis with excess hemin producing significant levels of oxidative stress, we speculate that hemin may modulate TSS pathway function

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