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Overall, 10 L of the reaction mixture was injected into a 150 4.6 mm Hypersil ODS column (Thermo Fisher Scientific, Waltham, MA USA) equilibrated with 30 mmol/L ammonium nitrate and 40 mmol/L ammonium formate buffer, pH 3.6 (buffer A)

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This was surprising considering the theoretical isoelectric point based on the amino acid sequence of the capsid structural proteins is 6.1 (Supplementary Figure S7)

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In the first, Atg7 and Atg10 mediate the conjugation of Atg12 to Atg5