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It includes Vitamin C, B-complex, B-12, and Magnesium, enhanced with a double dose of Glutathione for quicker and more pronounced benefits

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Nakamura, T., Arii, S., Monden, Z., Furutani, M., Takeda, Y., Imamura, M., Tominaga, M

- different GST isoenzymes exist in plants Often GSTs are the basis of herbicide selectivity: - crop plants (maize): high GST activity - competing weeds: low GST activity Herbicide detoxification by GSTs Triazines: atrazine, simazine Chloroacetanilides: acetochlor, alachlor, metolachlor Thiocarbamates: EPTC Nitrodiphenylethers: acifluorfen, fluorodifen Sulfonylureas: chlorimuron ethyl Others: chlorfenprop-methyl, metribuzin Transgenic tobacco Transformation of tobacco with a maize gene encoding the GST27 isoenzyme conferred tolerance to chloroacetanilide herbicides (Jepson et al., 1997) Herbicide safeners (antidotes) Wide range of synthetic compounds They increase the herbicide tolerance in crop plants (usually in cereals) Mode of action: Selective induction of GST isoenzymes participating in herbicide detoxification Other toxic GST substrates 1,2-dichloro-4-nitrobenzene 1-chloro-2,4-dinitrobenzene 4-nitropyridine-N-oxide p-nitrophenethyl-bromide p-nitrobenzyl chloride bromosulfophthalein Classical definition of GSTs: GSTs catalyze the conjugation of glutathione to a wide range of toxic and hydrophobic compounds large isoenzyme family predominantly cytosolic in maize: 1-2% of all soluble proteins endogenous, physiological substrates

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However, as host immunity mounts, IFN--mediated induction of ROS and reactive nitrogen intermediates (RNI) triggers phagosomal maturation leading to a subsequential drop in the pH (5.5 or lower)