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Periplasmic production via the pET expression system of soluble, bioactive human growth hormone

Membranes were developed using an Amersham Imager 600 (GE Healthcare Life Sciences) with SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34096)

This resulted in a.gff file that was visualised using TOUCAN ( [66] without showing the individual motifs

For example, the galectin 3 binding protein gene ( LGALS3BP ) exhibited a hypermethylated profile after supplementation, indicating potential downregulation and enhanced regulation of inflammatory responses

1n, Supplementary Data 3a-c)

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