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Dermal cells were digested in 0.35% collagenase I (#LS004197, Worthington, USA) for 20 min and then obtained by filtration and centrifugation

Parikh K, Zhou N, Hofstetter W, Mehran RJ, Rice DC, Sepesi B, Swisher S, Vaporciyan AA, Walsh G, Antonoff MB

Studies have shown that people can have high or normal B12 levels in the blood and actually show virtually no B12 in the spinal fluid, meaning the B12 is not being transported from the blood stream into the cells where it should be absorbed and used