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The analytes were eluted under gradient conditions at a flow rate of 0.20 mL min 1 with a linear ramp of methanol to 90% methanol at 19 min, which was maintained for 6 min before resetting to the original conditions

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The release of MMP enzymes by severe reactive astrocytes can impair BBB integrity and trigger the production of proinflammatory cytokines 52

Psoriatic arthritis, affecting up to 30% of psoriasis patients, can cause inflammatory joint pain, stiffness, swelling, and irreversible joint damage if untreated (Chimenti et al

GST tags Epitope tags such as glutathione-S-transferase (GST) are often used as tags for proteins for expression and purification applications