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In detail, PI3K induces PtdIns(3,4,5)P(3) to combine with the pleckstrin homology domain of msin1, blocking inhibition of the mTOR kinase domain by msin1, thereby activating mTORC2 [32, 33]

These findings strongly suggest that compounds derived from Heptaptera triquetra, particularly colladin, hold significant promise as natural therapeutic agents for the management of neurodegenerative and ocular diseases [155]

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For complex biological matrices, LC-MS/MS is preferred due to its high sensitivity and specificity, allowing for the detection of lysine and its metabolites without extensive derivatization by using HILIC columns or ion-pairing chromatography to handle the molecules high polarity