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Proteins were diluted to 25 g in Pierce RIPA Buffer lysis reagent with 1X Laemmli Sample Buffer (Bio-Rad) containing 10% -mercaptoethanol and incubated for 10 min at 70 C

Am J Gastroenterol 97:14381444 Lamarque D, Whittle BJ (2001) Increase in gastric intramucosal hydrogen ion concentration following endotoxin challenge in the rat and the actions of nitric oxide synthase inhibitors

For each sample, the number of cells of the reported type was divided by the total number of cells (excluding erythrocytes)

Vitamin B6 injection (pyridoxine) is essential for protein metabolism, energy production, and normal nervous system function

Now, youll see the powder start to dissolve