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Kinetics of association of serine proteinases with native and oxidized alpha-1-proteinase inhibitor and alpha-1-antichymotrypsin

As the RG7774 stock solution was 100% DMSO, the final concentration of DMSO per assay point was diluted to 0.1%

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14 FranssonA.KisielM.PirttilaK.PetterssonC.Videhult PierreP.LaurellG

At this concentration: 100mcg = 0.05mL = 5 units on insulin syringe 200mcg = 0.10mL = 10 units 250mcg = 0.125mL = 12.5 units Alternative concentration: Add 1mL water to 5mg vial for 5mg/mL (5000mcg/mL)

To address the reactivities of the redox-sensitive cysteinyl residues, we also analyzed the recombinant monothiol roGFP2 variants roGFP2 C151S and roGFP2 C208S and detected small but reproducible glutathionylation-dependent changes of fluorescence at both excitation wavelengths for roGFP2 C151S (SSG) and roGFP2 C208S (SSG) (Fig