Increasing evidence indicates that targeting mitochondrial dysfunction using antioxidants, iron chelators, GPX4 activators and mitophagy modulators effectively attenuates ferroptosis and preserves disc cell viability
yoelii 17XL was maintained by serial passage in mice
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[DOI] [PMC free article] [PubMed] [Google Scholar] 124.Silva K.V.C., Costa B.D., Gomes A.C., Saunders B., Mota J.F
10.1002/ptr.3388 124 JohnstonA.SmithP
(2) administering to a test animal a library of replicable genetic packages, each of which comprises a member of the library of experimentally generated (in vitro &/or in vivo) polynucleotides operably linked to a polynucleotide that encodes a display polypeptide, wherein the experimentally generated (in vitro &/or in vivo) polynucleotide and the display polypeptide are expressed as a fusion protein which is which is displayed on the the methods of the invention further involve: (4) reassembling (&/or subjecting to one or more directed evolution methods described herein) a nucleic acid that comprises at least one experimentally generated (in vitro &/or in vivo) polynucleotide obtained from a replicable genetic package recovered from the selected tissue with a further pool of polynucleotides to produce a further library of experimentally generated (in vitro &/or in vivo) polynucleotides
