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Tissue culture Each of the 11 non-herniated samples was divided into two small tissue blocks (~5 mm 3 ), which were cultured (37 C, 5% CO 2 ) in a Mat-Tek petridish containing Dulbeccos Modified Eagle Medium (DMEM) with 5 ml/l penicillin (Thermo Fisher Scientific, UK) and 2.5 ml/l Gibco amphotericin B (Thermo Fisher Scientific, UK)