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BFRs are also of concern due to their widespread occurrence in the environment and adverse effects on living organisms, including humans

For instance, TME cues 15,16 may not be clearly distinguishable from normal tissue, which would result in significant off-target effects in prodrugs

doi: 10.7759/cureus.9018

So that's step two

As such, individuals from the Kahemba region were recruited based on prior diagnosis of konzo by expert phenotyping of this disease, who were participants in the long-term clinical trials aimed at altering cassava processing to reduce konzo

Effects of combination of melatonin and dexamethasone on secondary injury in an experimental mice model of spinal cord trauma