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coli BL21 star (DE3) cells

Rugged skin seems lifeless and worn out because dead cells accumulate and scatter light

The second hexa-histadine tag of the protein was not utilized in these experiments, but is relevant and must be noted because Ni-NTA was used to purify the GST-GFP for several of the experiments being discussed

Here, we addressed our mechanistic models experimentally using ScGrx7 as a reference enzyme 11,12,18

Observed effects are dependent on concentration, cellular context, and experimental design, and are used to investigate molecular mechanisms rather than defined physiological outcomes

Conversely, KEAP1 downregulation (Supplementary Figure S6E) sensitised EGFRm/T790M PC9ER cells to erlotinib (Figure 3g), accompanied by the increased GSH levels (Figure 3h) and increased transcription of GSH-synthesising enzymes GCLC, GSR and GSS (Supplementary Figure S6F)