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Quantification of autophagy induction in microfilariae and cell lines through western blot analysis Brugia malayi mf and SF9, THP-1 and MDCK cells were tested for autophagy induction in response to the antibiotics by western blot

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The radioactive proteins synthesized are separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and detected by radioautography