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As above, daf12 and unc1 are synthetically lethal, but the lossoffunction allele of nhr1 allowed us to isolate triple mutants unc1

In skeletal muscle of UBE3B KO mice, there is an accumulation of BDK that leads to a decrease in BCKDH complex activity, but this does not correlate with changes in BCAA levels, indicating that BCKAs could be driven to other pathways (Ziegler et al., 2017), therefore BDK accumulation may disrupt the regulation of lipogenesis in developing neurons

Therefore we retrospectively analyzed the prospective observational data

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