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In a July 2023 article, three researchers at the Istanbul University studied the hearts of 24 cavaliers -- 6 in Stage A (the control group), 6 in Stage B1, 6 in Stage B2, and 6 in Stage C -- to determine the VLAS (Vertebral Left Atrial Size, using x-rays of the dogs' hearts) and compare those VLAS measurements to echocardiographic measurements of the 2019 ACVIM stages of mitral valve disease (MVD)

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