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Principe M, Borgoni S, Cascione M, Chattaragada MS, Ferri-Borgogno S, Capello M, et al

The FT-IR technique also identifies the amorphous form and distinguishes it from the previously known polymorphs, as shown in Figure 1

Appetite 51, 233243

Determination of free thiol groups using Ellmans reagent (DTNB assay) To quantify accessible thiol groups, 200 l of purified Chi proteins (0.26 mg/ml) were incubated with 5 mM reducing agent (DTT or -mercaptoethanol) for 30 min at 37 C