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Nuclei were further purified by centrifugation at 1,000 g for 5 min at 4 C through a 1 M sucrose cushion (3 mM MgAc, 10 mM Tris-HCl, pH 8) and then stained with a PCM1 antibody in nuclei stain buffer (DPBS, 1% BSA, 0.2% Igepal CA-630, 1 mM EDTA)

Bromopyridazinedione-mediated protein and peptide bioconjugation

doi: 10.1093/eep/dvac029 124 TownsendJ.PengZ.KizilaslanM.TaylorT.YangZ.AhsanN.et al

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155 Hepatic Storage Function Because individual cells are unable to store a sufficient supply of energy-rich carbohydrate substrates, the liver serves as the major site for their storage