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Description

Data-independent acquisition mass spectrometry in severe rheumatic heart disease (RHD) identifies a proteomic signature showing ongoing inflammation and effectively classifying RHD cases

However, isotope-labeled IS are often expensive or unavailable for certain compounds and using a single isotope-labeled IS for multiple structurally diverse compounds is inappropriate due to differences in retention times, matrix effects, ion suppression, fragmentation patterns, and ionization efficiencies