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This involved perfusing the brains with phosphate-buffered saline followed by 4% paraformaldehyde, sectioning them into eight-micrometer coronal slices, and visually confirming the cannulas location within the left lateral ventricle [1]

And specially trained phlebotomists may be permitted to give injections in certain settings

Flies with Usp8 knockdown exhibited disrupted locomotor activity rhythms and displayed either arrhythmic or lengthened periods, displaying the role of Usp8 in maintaining circadian timing (Luo et al., 2012)