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The percentage of PI-positive apoptotic cells was quantified

However, it is important to keep in mind that CLE visualizes a basolateral (subepithelial) to apical (luminal) flux of a small molecule (fluorescein) of which the correlation to luminal to subepithelial passage of luminal compounds, which is hypothesized to trigger a mucosal immune response and symptoms, is still unclear

The lipid-clearing effects of the glucagon analogue were independent of the PDE4/cAMP/PKA axis that governed energy expenditure, meaning the fat-lowering and calorie-burning arms of glucagon action are at least partly separable at the molecular level

Miller DM, Buettner GR, Aust SD

2021 Dec 1;157(12):1456-1465

5 Conclusion In summary, the addition of 0.75 M Vit A and 2 M Lup to bucks semen cryopreservation extenders significantly improved post-thawing sperm motility parameters, plasma membrane integrity, and antioxidant capacity, while reducing the proportion of apoptotic spermatozoa and may improve fertilization and early embryonic development in vitro , although further studies are needed to confirm this effect